Specification :

Neomycin ELISA test Kit
1. Principle
This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated
on the micro-well stripes. The Neomycin residues in the sample and the coupling antigen precoated
on the micro-well stripes compete for the anti-Neomycin antibody. After the addition of the
enzyme conjugate, the TMB substrate is added for coloration. The optical density ( OD) value of the
sample has a negative correlation with the Neomycin in it. This value is compared to the standard
curve and the Neomycin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.5 ppb
Detection limit
Honey................................................................. ..................................... 10 ppb
Chicken/ liver, pork/ liver, fish, shrimp....................................................... 10 ppb
Recovery rate
Chicken, pork, fish, shrimp....................................................................85 ± 15%
Liver.................................................................. .....................................70± 10%
Cross-reaction rate
Neomycin............................................................... ................................... 100%
Streptomycin........................................................... ................................ < 0.1%
Gentamicin............................................................. ................................. < 0.1%
Dihydrostreptomycin.................................................... ........................... < 0.1%
Kanamycin.............................................................. ................................ < 0.1%
Tobramycin............................................................. .................................< 0.1%
Sisomicin.............................................................. ...................................< 0.1%