Specification :

Chloramphenicol
ELISA
test
Kit
1.
Principle
This test kit is based on the competitive enzyme immunoassay for the detection of
Chloramphenicol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The
Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes
compete for the anti-Chloramphenicol antibody. After the addition of the enzyme conjugate, the
TMB substrate is added for coloration. The optical density ( OD) value of the sample has a negative
correlation with the Chloramphenicol in it. This value is compared to the standard curve and the
Chloramphenicol concentration is subsequently obtained.
2.
T
echnical
specifications
S
ensitivity
:
0.05 ppb
D
etection
limit
Intestine.............................................. ....................................................0.1 ppb
Honey ............................................................................ ....................... 0.15 ppb
Milk.................................................................... ................................... 0.05 ppb
Urine and serum....................................................................... ..............0.1 ppb
Chicken/ liver, pork/ liver, fish, shrimp....................................................0.05 ppb
R
ecovery
rate
Chicken/ liver, pork/ liver, fish, shrimp..................................................80% ± 10%
Honey ............................................................................ .....................70% ± 10%
Milk........................................... ..........................................................85% ± 15%
Cross
-rea ction
rate
Chloramphenicol ............................................................................ ........... 100%
Thiamphenicol ............................................................................ .............< 0.1%
Florfeniol............................................................. .................................... < 0.1%