Specification :

Source: E.coli;
M.W.: The recombinant Protein G, containing 196 amino acid residues which is amino acid 190-384 of the Streptococcus sp, has a predicted molecular mass of approximately 21.6 kDa. But it migrates with an apparent molecular mass of 32 kDa in SDS-PAGE;
Purity: >95% by SDS-PAGE analyses;
Endotoxin Level: Less than 0.1 EU/ug of Protein G as determined by LAL method;
Formulation: Lyophilized with no additive;
Specificity: 1. Binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a;
2. Does not bind to human IgM, IgD and IgA.
Applications: Protein G binds to the constant region of many species of immunoglobulin G. It can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. GenicBio's Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity;